The Captain of the Duloc Guards is first seen in a forest with other knights putting various fairy tale creatures in wagons and rewarding the villagers who are turning them in. He awards five shillings to Geppetto for handing in Pinocchio to the guards, and he almost awards ten shillings to the Old Woman if she could prove Donkey could talk, which she couldn't. As the knights were going to drag her away, the woman kicks a jar containing Tinkerbell, who makes Donkey fly and escape. The Captain and his men chases Donkey into the forest until he runs into Shrek. The Captain orders his men to arrest the both of them, but they are easily frightened away by Shrek.
Later, in the Duloc Castle, the Captain arrives and presents Lord Farquaad with the Magic Mirror. The Magic Mirror presents three "eligible bachelorettes" whom Farquaad can marry, in which the Captain suggested him to take bachelorette number two. Farquaad ends up picking number three, who is Princess Fiona. He orders the Captain to prepare his finest men for a tournament, which will determine who is worthy to rescue Fiona from the tower.
- What have you got?
- Five shillings for the possessed toy. Take it away.
- Right. Well, that's good for ten shillings, if you can prove it.
- That's it. I've heard enough. Guards!
- Get her out of my sight.
- He can talk!
- Seize him!
- The Captain of the Guards is voiced by Jim Cummings.
- His real name is never known.